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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which results in chromosome instability, and thus might add to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential function in promoting cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have been explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information will not be but offered.
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Blocking peptides are peptides that bind especially to the focus on antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best success are...
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This information outlines further more possible optimization of mobile and immunoassay parameters to ensure the absolute best results are...
Antibodies bound to the blocking peptide no longer bind to the epitope around the target protein. By evaluating the staining within the blocked antibody vs . the antibody alone, one can see which staining is certain.
Goal Info Cyclin E belongs towards the highly conserved cyclin family, whose users are characterised by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features to be a regulatory subunit of CDK2, whose activity is required for mobile cycle here G1/S transition. This protein accumulates within the G1-S phase boundary and is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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